Stimulation of Na⁺/K⁺-ATPase with an Antibody against Its 4^th Extracellular Region Attenuates Angiotensin II-Induced H9c2 Cardiomyocyte Hypertrophy via an AMPK/SIRT3/PPARγ Signaling Pathway

Figure 2

Effects of DR-Ab on Ang II-induced intracellular ROS generation in H9c2 cells. DR-Ab (2 μM) was given 30 min before treatment with Ang II (100 nM) for 48 h. (a–c) Representative immunofluorescence image (a) and group data (b, c) showing that DR-Ab decreased ROS generation caused by Ang II. Red: DHE staining (a, upper). Scale bar, 100 μm. Green: DCFH-DA staining (a, lower). Scale bar, 50 μm. . (d) Effect of DR-Ab on the protein level of two subunits of NADPH oxidase: p22^phox and p47^phox. . versus control group, ^† versus Ang II alone group.

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