Stimulation of Na⁺/K⁺-ATPase with an Antibody against Its 4^th Extracellular Region Attenuates Angiotensin II-Induced H9c2 Cardiomyocyte Hypertrophy via an AMPK/SIRT3/PPARγ Signaling Pathway

Figure 6

Western blotting analysis showing that DR-Ab stimulated AMPK/Sirt-3/PPARγ signaling pathway. (a) Time-course study showing the effect of DR-Ab on AMPK phosphorylation. (b–d) DR-Ab reversed the effect of Ang II on p-AMPK (b), PPARγ (c), & Sirt-3 (d). . (e) 3-TYP eliminated DR-Ab effect on the PPARγ level in Ang II-treated cells. . (f, g) Compound C abolished the effect of DR-Ab on the protein expression of PPARγ (f) and Sirt-3 (g) in Ang II-treated cells. . versus control group, ^† versus Ang II alone group, ^‡ versus Ang II+ DR-Ab.

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