Tricetin Protects Rat Chondrocytes against IL-1β-Induced Inflammation and Apoptosis

Figure 3

Effects of tricetin on the expression of matrix metalloproteinase- (MMP-) 1, MMP-3, and MMP-13, as determined by real-time PCR (RT-PCR) (a) and western blot (b). Chondrocytes pretreated with 10 or 20 μM tricetin for 3 h were cultured with interleukin- (IL-) 1β (10 ng/ml) for 24 h. Each column represents the from triplicate samples in three independent experiments. IL-1β (10 ng/ml) alone significantly increased MMP-1, MMP-3, and MMP-13 expression compared to the untreated group (). Tricetin (10 or 20 μM) decreased IL-1β-induced MMP production. and ; NS: not significant.

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