Tricetin Protects Rat Chondrocytes against IL-1β-Induced Inflammation and Apoptosis

Table 1

Real-time PCR primers and conditions.

Gene	GenBank accession	Primer sequences (5 to 3)	Size (bp)	Annealing (°C)
GAPDH	NM_017008.4	GAAGGTCGGTGTGAACGGATTTG	127	60
		CATGTAGACCATGTAGTTGAGGTCA
MMP-1	NM_001134530.1	GCTTAGCCTTCCTTTGCTGTTGC	136	60
		GACGTCTTCACCCAAGTTGTAGTAG
MMP-3	NM_133523	CTGGGCTATCCGAGGTCATG	77	60
		TGGACGGTTTCAGGGAGGC
MMP-13	NM_133530	CAACCCTGTTTACCTACCCACTTAT	85	60
		CTATGTCTGCCTTAGCTCCTGTC
COX-2	S67722	CGCATTCTTTGCCCAGCACTTCACT	190	60
		CACCTCTCCACCGATGACCTGATA
iNOS	NM_012611.3	GCTCGGGCTGAAGTGGTATGC	127	60
		GAAGTCTCGGACTCCAATCTCGGT
Bcl2	L14680	GGCTACGAGTGGGATACTGGAGAT	86	60
		CTCTCAGGCTGGAAGGAGAAGATG
Bax	NM_017059	CCCCAGGACGCATCCACCAA	112	60
		GGGAGTCTGTATCCACATCAGCAA