Maggot Extracts Alleviate Inflammation and Oxidative Stress in Acute Experimental Colitis via the Activation of Nrf2

Figure 1

The maggot extracts are capable of suppressing inflammation and oxidative stress in LPS-stimulated RAW 264.7 cells via activation of Nrf2 in a dose-dependent manner. (a) Cells were treated with the maggot extracts for 4 h at various doses as indicated followed by analysis of the induction of Nrf2 and its downstream protein HO-1 with western blotting. GAPDH was used as an internal reference. (b) Cells were treated with a combination of LPS, the maggot extracts (4 μg/μl), or ML385 (3 μM) as indicated followed by analysis of IκB, NFκB p65, IL6, IL-1β, TNF-α, Nrf2, HO-1, Keap1, p22-phox, and gp91-phox by western blotting. GAPDH was used as an internal reference. Representative data are shown. (c–l) Western blot bands shown in (b) were analyzed by densitometry. The data are shown as the . , , and .

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