Cherry Anthocyanins Regulate NAFLD by Promoting Autophagy Pathway

Figure 5

Colocalization of lysosomes or autophagosome with lipid droplets in HepG2 cells. (a) Double labeling of oil droplets (Nile Red) and lysosomes (LysoTracker Green) showing increased colocalization of lysosomes with cellular lipids (yellow dots) upon CACN treatment versus OA-alone-treated cells. (b) Double labeling of oil droplets (Nile Red) and autophagosomes (DAPG) showing increased colocalization of autophagosomes with cellular lipids (yellow dots) upon CACN treatment versus OA-alone-treated cells.

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