Rosiglitazone Suppresses Calcium Oxalate Crystal Binding and Oxalate-Induced Oxidative Stress in Renal Epithelial Cells by Promoting PPAR-γ Activation and Subsequent Regulation of TGF-β1 and HGF Expression

Figure 2

RSG promoted the activity of MDCK cells by inhibiting TGF-β1/Smad and activating the HGF/c-Met pathway. (a–c) Protein expression levels of TGF-β1, HGF, c-Met, p-Met, PPAR-γ, p-PPAR-γ, Smad7, Smad2, Smad3, and pSmad2 3 were analyzed using Western blotting after treatment for 1, 2, and 4 hours. The relative protein levels were normalized to the GAPDH level. (d) Western blot assay of HGF after siRNA transfection. (e, f) siRNA-mediated knockdown of HGF led to significant inhibition of the antioxidant capacity of RSG. The quantitative results are expressed as the of three experiments. N: normal; Ox: oxalate; R: rosiglitazone; G: GW9662; H: hour. versus the control group; ^### versus the 0.5 mM oxalate group.

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