FGF21 Mediates Mesenchymal Stem Cell Senescence via Regulation of Mitochondrial Dynamics

Figure 1

Characterization of BM-MSCs replicative senescence. (a) Representative images of BM-MSC morphology under light microscopy at passage 4 (P4), passage 8 (P8) and passage 12 (P12) (i). The cell size of MSCs was calculated at P4, P8, and P12 (ii). (b) Representative images of SA-β-gal staining in BM-MSCs at P4, P8, and P12 (i). The SA-β-gal-positive cells at P4, P8, and P12 were calculated and presented as percentage of the total cells (ii). (c) Representative images of Ki-67 staining in BM-MSCs at P4, P8, and P12 (i). The Ki-67-positive cells at P4, P8, and P12 were calculated and presented as percentage of the total cells (ii). (d) Western blotting and quantitative analysis of the level of p53 and p21 proteins in BM-MSCs at P4, P8, and P12 (i, ii). Data are expressed as with per group. . Scale  μm.

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