FGF21 Mediates Mesenchymal Stem Cell Senescence via Regulation of Mitochondrial Dynamics

Figure 6

Overexpression of FGF21 in aged MSCs reduces cell senescence. (a) Representative images of SA-β-gal staining in BM-MSCs isolated from young and aged donors (i). Percentage of SA-β-gal-positive senescent cells in young MSCs and aged MSCs (ii). (b) Western blotting and quantitative analysis of the level of FGF21, p53, and p21 in young MSCs and aged MSCs (i, ii). (c) Representative images of SA-β-gal staining in aged BM-MSCs transfected with control lentivirus or FGF21 lentivirus (i). Percentage of SA-β-gal-positive senescent cells in aged BM-MSCs transfected with control lentivirus or FGF21 lentivirus (ii). (d) Western blotting and quantitative analysis of the level of FGF21, p53, and p21 in aged BM-MSCs transfected with control lentivirus or FGF21 lentivirus (i, ii). (e) The proposed mechanisms involved in FGF21 mediates MSC senescence via regulation of mitochondrial dynamics. Data are expressed as with per group. . Scale  μm.

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