Research Article
Nicorandil Attenuates LPS-Induced Acute Lung Injury by Pulmonary Endothelial Cell Protection via NF-κB and MAPK Pathways
Figure 4
Nico decreased LPS-induced HPAEC apoptosis. (a) Effects of Nico on the viabilities of HPAECs were assessed by CCK-8. (b) Hoechst 33342 staining showed that Nico decreased HPAEC apoptosis induced by LPS, and Gli abolished the effects. (c) Nico pretreatment markedly inhibited the expression of c-caspase-3, caspase-9, and CHOP in HPAECs stimulated with LPS, and Gli abolished the effects. Data were shown as of at least three independent experiments performed in triplicate. Statistically significant differences: versus the control group; versus the LPS group; versus the LPS+Nico group. Scale bar: 25 μm.
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