Nicorandil Attenuates LPS-Induced Acute Lung Injury by Pulmonary Endothelial Cell Protection via NF-κB and MAPK Pathways

Figure 8

Treatment with Nico attenuated oxidative stress induced by LPS. (a) Intracellular ROS was evaluated by DCFH-DA fluorescence (green) imaging which revealed that Nico reduced the ROS production induced by LPS and Gli blunted the alternation. (b, d) Western blotting analysis and immunofluorescence staining showed that pretreatment with Nico decreased the overexpression of Nox4 after LPS stimulation. (c) ELISA showed that Nico attenuated the activation of Nox4. The effects could be blocked by Gli. (b, e) Western blotting and immunofluorescence staining revealed that pretreatment with Nico induced a higher expression of MnSOD compared to the LPS group. Data were shown as of at least three independent experiments performed in triplicate. Statistically significant differences: versus the control group; versus the LPS group; versus the LPS+Nico group. Scale bar: 25 μm.

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