Bovine Herpesvirus 1 Productive Infection Led to Inactivation of Nrf2 Signaling through Diverse Approaches

Figure 7

BoHV-1 affected the association between Nrf2 and LaminA/C. MDBK cells in 60 mm dishes were mock infected or infected with BoHV-1 for 24 h. Nucleus proteins were purified using commercial nuclear protein purification kit (Beyotime Biotechnology, cat# P0027). IP was performed using antibodies against Nrf2 (a), LaminA/C (b), Nrf2 and unrelated IgG (c), and LaminA/C and IgG (d) (R: rabbit; M: mouse). Then, Western blots were performed using corresponding antibodies. (e) MDBK cells in 60 mm dishes were mock treated or treated with Trolox (1 mM) for 2 h. Nucleus proteins were purified using commercial nuclear protein purification kit (Beyotime Biotechnology, cat# P0027), and LaminA/C was detected by Western blots.

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