BCL6 Attenuates Proliferation and Oxidative Stress of Vascular Smooth Muscle Cells in Hypertension

Figure 1

Effects of BCL6 overexpression (OE) on Ang II-induced VSMC proliferation. VSMCs were treated with empty plasmid or BCL6 plasmid (1 μg/mL) for 24 h followed by PBS or Ang II (100 nM) treatment for 48 h. (a) VSMC proliferation was determined with CCK-8 assay. . (b) Percentage of EdU-positive cells. (c) Representative images showing EdU-positive cells measured with Edu incorporation assay. Blue fluorescence shows cell nuclei and red fluorescence stands for cells with DNA synthesis. (d) PCNA protein expression. (e) BCL6 protein expression. Values are mean ± SE. vs. PBS; vs. Ctrl. in (a) and (b); in (c) and (d).

(a)

(b)

(c)

(d)

(e)