Pasteurized Orange Juice Rich in Carotenoids Protects Caenorhabditis elegans against Oxidative Stress and β-Amyloid Toxicity through Direct and Indirect Mechanisms

Figure 3

Effect of pasteurized juice from ‘Bahia’ and ‘Cara Cara’ oranges on the expression of antioxidant and detoxification genes. Analysis of gcs-1::GFP (a), gst-4::GFP (b), sod-3::GFP (c), and hsp-16.2::GFP (d) fluorescent expression levels. Transgenic worms were treated or not with 2% ‘Bahia’ or 2% ‘Cara Cara’ juice for 48 h. After this period, animals were submitted to stress conditions. For gcs-1::GFP (a), gst-4::GFP (b), and sod-3::GFP (c); the stress condition was incubation for 1 h on 10 mM TBHP. For hsp-16.2::GFP, the stress condition was incubation for 1 h at 35°C. Photographs were taken on a fluorescence microscope, and GFP fluorescence signals were measured using NIH ImageJ software. The results represent mean GFP of values. by a two-tailed Student’s -test compared to the untreated group, by a two-tailed Student’s -test for 2% ‘Bahia’ with 2% ‘Cara Cara’ juice.

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