Neuroprotection of Indole-Derivative Compound NC001-8 by the Regulation of the NRF2 Pathway in Parkinson’s Disease Cell Models

Figure 1

DAergic differentiation of SH-SY5Y cells. (a) Experiment flow chart of the differentiation of DAergic neurons from SH-SY5Y cells. Cells were incubated in the differentiation medium containing TPA (120 nM) and NC001-8 (100 nM) for 14 days. (b) Representative fluorescence microscopy images of differentiated DAergic neurons after 14 days of induction. Neurons expressed TUBB3 (green) and TH (red). (c) Drug cytotoxicity was estimated by using MTT assay, and the concentration of NC001-8 needed to maintain 90% of the growth was determined to be 100 nM. (d) Differentiated cells under NC001-8 treatment expressed TUBB3 and TH on day 14. (e) The percentage of TH-positive cells and (f) quantification of neurite outgrowth of DAergic neurons under NC001-8 treatment. Data were normalized to the treatment with TPA only and were represented as the of three independent experiments. DAergic: dopaminergic; MTT: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; TH: tyrosine hydroxylase; TPA: 12-O-tetradecanoyl-phorbol-13-acetate; TUBB3: tubulin beta 3 class III.

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