Neuroprotection of Indole-Derivative Compound NC001-8 by the Regulation of the NRF2 Pathway in Parkinson’s Disease Cell Models

Figure 3

Enhancement of the NRF2 antioxidative pathway by NC001-8 in SH-SY5Y-differentiated DAergic neurons. (a) Heat map was representative for ROS-associated genes in 3 groups analyzed by clustering 3.0 software. Data were normalized to cells with no treatment. (b, c) Analysis of qRT-PCR and (d) Western blot of NRF2 and NQO1 in DAergic neurons treated with MPP⁺ and/or NC001-8/or tBHQ (25 μM for 6 hours, as a positive control for NRF2 induction [41]). Data were normalized to GAPDH and compared to cells with no treatment (, independent assays). (e) Nucleus translocation of NRF2 in DAergic neurons treated with MPP⁺ and/or NC001-8/or tBHQ (25 μM for 6 hours). Data were normalized to H3 and compared to cells with no treatment (, independent assays). . tBHQ: tert-butylhydroquinone; qRT-PCR: quantitative reverse transcription-polymerase chain reaction; DAergic: dopaminergic; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; MPP⁺: 1-methyl-4-phenylpyridinium; NRF2: nuclear factor erythroid 2-related factor 2; NQO1: NAD(P)H dehydrogenase, quinone 1.

(a)

(b)

(c)

(d)

(e)