Neuroprotection of Indole-Derivative Compound NC001-8 by the Regulation of the NRF2 Pathway in Parkinson’s Disease Cell Models

Figure 5

Neuroprotective and antioxidative effects of NC001-8 in PD-iPSC-derived DAergic neurons. (a) Scheme of the experimental design. DAergic neurons derived from PD-iPSCs were treated with NC001-8 (100 nM) before H₂O₂ (100 μM) treatment for 8 hrs. (b) Western blot of NRF2, NQO1, and cleaved caspase 3 in DAergic neurons derived from NC- and PD-iPSCs treated with H₂O₂ and/or NC001-8. Data were normalized to GAPDH and compared to NC1 with no treatment. (c) The production of ROS in DAergic neurons treated with H₂O₂ and NC001-8. Data were normalized to NC1 with no treatment (over 500 cells were counted). Above these results were presented as the from three different passages; . NC-iPSC: induced pluripotent stem cells derived from a healthy volunteer; PD-iPSC: induced pluripotent stem cells carrying a PARKIN ex5del mutation; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; NRF2: nuclear factor erythroid 2-related factor 2; NQO1: NAD(P)H dehydrogenase, quinone 1; ROS: reactive oxygen species.

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