Melatonin and Docosahexaenoic Acid Decrease Proliferation of PNT1A Prostate Benign Cells via Modulation of Mitochondrial Bioenergetics and ROS Production

Figure 2

Lipid accumulation. (a) Detection of lipids (green) in PNT1A cells with BODIPY. Cells were incubated with 100 μM of DHA and 1 μM of MLT for 48 h before the dye. Images of left column were captured with 10x objective and right column (insets) with 40x. (b) Lipid storage quantification. C: control (vehicle incubation); DHA: incubation with 100 μM of DHA for 48 h; MLT: incubation with 1 μM of melatonin for 48 h; DM: coincubation with 100 μM of DHA and 1 μM of melatonin for 48 h. Statistical analysis: (A) different from C; (B) different from DHA; (C) different from MLT. was considered statistically different. At least four hundred cells per treatment from three consecutive passages were analyzed. Values show the mean of fluorescent units per cell and SEM.

(a)

(b)