Research Article
Melatonin and Docosahexaenoic Acid Decrease Proliferation of PNT1A Prostate Benign Cells via Modulation of Mitochondrial Bioenergetics and ROS Production
Figure 5
Morphometric analysis of mitochondria. (a) Mitochondria labeling with MitoTracker, (b) area (μm2), (c) perimeter (μm), and (d) mitochondrial membrane potential assessed with Safranin O. Images captured with 40x objective with an inverted fluorescence microscope for morphological analysis. Legend: C: control (vehicle incubation); DHA: incubation with 100 μM of DHA for 48 h; MLT: incubation with 1 μM of melatonin for 48 h; DM: coincubation with 100 μM of DHA and 1 μM of melatonin for 48 h; ADP: adenosine diphosphate; OLG: oligomycin; AA: antimycin A. At least 100 cells from consecutive passages were considered for statistical analysis. Values show the mean and SEM. Statistical analysis: (A) different from C; (B) different from DHA; (C) different from MLT. was considered statistically different.
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