Research Article

Active Constituent in the Ethyl Acetate Extract Fraction of Terminalia bellirica Fruit Exhibits Antioxidation, Antifibrosis, and Proapoptosis Capabilities In Vitro

Figure 7

Effect of GA on HSC-T6 apoptosis was measured via annexin V-PI staining (200x). (a1, a2) Control. (b1, b2) Cells were incubated with 12.5 μg/mL GA. (c1, c2) Cells were incubated with 25 μg/mL GA. (d1, d2) Cells were incubated with 50 μg/mL GA. Cells stained only with green by annexin V are in the early stage of apoptosis (blue arrow), cells stained only in red by PI are damaged cells (yellow arrow), and cells stained in both green and red are in advanced apoptosis or in necrosis (white arrow).