[Retracted] Purple Sweet Potato Color Attenuates Kidney Damage by Blocking VEGFR2/ROS/NLRP3 Signaling in High-Fat Diet-Treated Mice

Figure 4

α-Lipoic acid decreases ROS-triggered inflammation in the kidney of HFD-treated mice. (a) ROS levels were assessed by dihydroethidium (DHE) staining. (b) DHE fluorescence intensity was measured by the IPP, and results are expressed by the ratio of the integrated optical density of the DHE-positive area to the DAPI. (c) Representative immunoblots for NLRP3, procaspase 1, cleaved caspase 1, pro-IL1β, cleaved IL1β, and β-actin. (d) Relative density analysis of NLRP3, cleaved caspase 1, and cleaved IL1β protein bands. Relative densities are expressed as the ratio of NRLP3 to β-actin, cleaved caspase 1 to procaspase 1 (expressed as cleaved/procaspase 1), and cleaved IL1β to Pro-IL 1β (expressed as cleaved/pro-IL1β). (e) Representative immunoblots for cytoplasmic NF-κB p65 (C-NF-κB p65), nuclear NF-κB p65 (N-NF-κB p65), β-actin, and histone H3 (H3). (f) Relative density analysis of the C-NF-κB p65 and N-NF-κB p65 protein bands. Relative densities are expressed as the ratio of C-NF-κB p65 to β-actin and N-NF-κB p65 to H3. (g) Representative immunoblots for TNFα, IL6, iNOS, and β-actin. (h) Relative density analysis of TNFα, IL6, and iNOS protein bands. Relative densities are expressed as the ratio of TNFα, IL6, and iNOS to β-actin. All values are expressed as mean ± SEM ((a–h), ). vs. the CD vehicle group, and vs. the HFD vehicle group.

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