Research Article
Cell Type- and Exposure-Specific Modulation of CD63/CD81-Positive and Tissue Factor-Positive Extracellular Vesicle Release in response to Respiratory Toxicants
Figure 3
Effect of silica particles, PM2.5, PM10, and the PM vehicle control (v.c.) on bronchial epithelial cells. BEAS-2B cells were exposed for 24 h to different concentrations of the 4 stimuli. Silica concentrations are given in ×106 μm2/cm2, and PM concentrations in m3/ml. (a) Cell metabolic activity determined by the MTT assay, ; (b) cell surface thiols after 24 h exposure to silica or 1.5 m3/ml PM, . (c) ROS-generating capacity of PM2.5 and PM10 as determined by ESR. Paired PM2.5 and PM10 samples from the same sampling location are connected by a line. (d) Release of CD63+CD81+ EVs determined by bead-based flow cytometry, . compared to the unexposed control (100%); # for PM10 compared to PM2.5.
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