Research Article

DpdtbA-Induced Growth Inhibition in Human Esophageal Cancer Cells Involved Inactivation of the p53/EGFR/AKT Pathway

Figure 3

Apoptosis analysis of ESC cell lines via flow cytometer. DpdtbA was incubated with the cells for 24 h. All attached cells were collected and double stained with annexin V and propidium iodide (PI) using a kit from Dojindo Laboratories following the manufacturer’s instructions. (a) Kyse 450 cells and (b) Kyse 150 cells. The condition was as indicated in the figure.
(a)
(b)