ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells

Figure 3

H₂O₂ inhibits progesterone synthase expressions and progesterone production in pGL. (a) pGL were treated for 24 h with vehicle control or different concentrations (0.2 or 0.4 mM) of H₂O₂, and StAR mRNA levels were examined by RT-qPCR. (b) pGL were treated for 24 h with vehicle control or different concentrations (0.2 or 0.4 mM) of H₂O₂, and CYP11A1 mRNA levels were examined by RT-qPCR. (c) pGL were treated for 24 h with vehicle control or different concentrations (0.2 or 0.4 mM) of H₂O₂, and 3β-HSD mRNA levels were examined by RT-qPCR. (d) pGL were treated for 24 h with vehicle control or different concentrations (0.2 or 0.4 mM) of H₂O₂, and progesterone accumulation levels in culture medium were assayed by ELISA. These results are expressed as the of at least 3 independent experiments, and values labeled with different letters are significantly different ().

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