ROS-Induced GATA4 and GATA6 Downregulation Inhibits StAR Expression in LPS-Treated Porcine Granulosa-Lutein Cells

Table 1

Primers used in this study.

Gene	Accession no.	Primer sequences (5-3)	Annealing	Length
β-Actin	XM_003357928.2	F: CTTCCTGGGCATGGAGTCC	60°C	201 bp
		R: GGCGCGATGATCTTGATCTTC
StAR	AY368628.1	F: CATTACCATCTACTCCCAGC	60°C	109 bp
		R: AACCCGTATCTTTCTTGTCAG
CYP11A1	NM_214427.1	F:GTCCCATTTACAGGGAGAAGCTCG	60°C	182 bp
		R: GGCTCCTGACTTCTTCAGCAGG
3β-HSD	NM_001004049.1	F: TTCCTGGCAAGTATTTCTCGG	60°C	110 bp
		R: TCCAGCAACAAGTGGACGAT
NR5A1	NM_214179.1	F: CTGCCTCAAGTTCCTCATTCTC	60°C	122 bp
		R: GGTAGTGGCACAGGGTGTAATC
GATA4	NM_214293	F: TGAAGCTCCATGGTGTCCC	60°C	150 bp
		R: CTGCTGGAGTTGCTGGAAG
GATA6	NM_214328	F: AGAAACGCCGAGGGTGAAC	60°C	110 bp
		R: CGTTTCCTGGTCTGAATTCCC