Ziziphus spina-christi Leaf Extract Suppressed Mercury Chloride-Induced Nephrotoxicity via Nrf2-Antioxidant Pathway Activation and Inhibition of Inflammatory and Apoptotic Signaling

Table 1

Primer sequences of genes analyzed by using real-time PCR.

Name	Accession number	Sense (5-3)	Antisense (5-3)
Gapdh	NM_017008.4	GCATCTTCTTGTGCAGTGCC	GATGGTGATGGGTTTCCCGT
Gclc	NM_012815.2	TGTCGCTGGGGAGTGATTTC	GGTCAGACTCGTTGGCATCA
Gclm	NM_017305.2	AGTGGGCACAGGTAAAACCC	CAATGCTTCCTGTGAGTGCG
Nqo1	NM_017000.3	ATTGTATTGGCCCACGCAGA	TCATATCCCAGGCCACCTGA
Sod2	NM_001270850.1	AGCTGCACCACAGCAAGCAC	TCCACCACCCTTAGGGCTCA
Cat	NM_012520.2	TCCGGGATCTTTTTAACGCCATTG	TCGAGCACGGTAGGGACAGTTCAC
Gpx1	NM_017006.2	CGGTTTCCCGTGCAATCAGT	ACACCGGGGACCAAATGATG
Gsr	NM_053906.2	TGCACTTCCCGGTAGGAAAC	GATCGCAACTGGGGTGAGAA
Nfe2l2	NM_031789.2	GGTTGCCCACATTCCCAAAC	GGCTGGGAATATCCAGGGC
Hmox1	NM_012580.2	GCGAAACAAGCAGAACCCA	GCTCAGGATGAGTACCTCCCA
Havcr1	NM_173149.2	TGGCACTGTGACATCCTCAGA	GCAACGGACATGCCAACATA
Bcl2	NM_016993.1	CTGGTGGACAACATCGCTCTG	GGTCTGCTGACCTCACTTGTG
Bax	NM_017059.2	GGCGAATTGGCGATGAACTG	ATGGTTCTGATCAGCTCGGG
Casp3	NM_012922.2	GAGCTTGGAACGCGAAGAAA	TAACCGGGTGCGGTAGAGTA
Tnfα	NM_012675.3	GGCTTTCGGAACTCACTGGA	CCCGTAGGGCGATTACAGTC
Il1β	NM_031512.2	GACTTCACCATGGAACCCGT	GGAGACTGCCCATTCTCGAC
Nos2	NM_012611.3	GTTCCTCAGGCTTGGGTCTT	TGGGGGAACACAGTAATGGC

Gapdh: glyceraldehyde 3-phosphate dehydrogenase; Sod2: manganese-dependent superoxide dismutase (MnSOD); Cat: catalase; Gclc: glutamate-cysteine ligase catalytic subunit; Gclm: glutamate-cysteine ligase modifier subunit; Gpx1: glutathione peroxidase; Gsr: glutathione reductase; Nqo1: NAD(P)H quinone dehydrogenase 1; Nfe2l2: nuclear factor-erythroid 2-related factor 2; Hmox1: heme oxygenase 1; Havcr1 (Kim1): hepatitis A virus cellular receptor 1 (kidney injury molecule-1); Bcl2: B-cell lymphoma 2; Bax: Bcl-2-like protein 4; Tnfα: tumor necrosis factor alpha; Il1β: interleukin-1 beta; Nos2: nitric oxide synthase 2.