Autophagy: A Player in response to Oxidative Stress and DNA Damage

Figure 3

Autophagic pathway induction evaluated through flow cytometry in the U937 cell line after a 24 h treatment with 67 μM bleomycin (a), 0.5 μM bortezomib (b), 1.9 mM ethyl methanesulphonate (c), and 1.3 μM menadione (d). The accumulation of autophagic vesicles was induced through the treatment with the autophagy inhibitor, 10 μM chloroquine. 20000 events were collected for every tested condition through the NovoCyte Flow Cytometer (ACEA, Biosciences Inc.).

(a)

(b)

(c)

(d)