Tetramethylpyrazine Attenuates the Endotheliotoxicity and the Mitochondrial Dysfunction by Doxorubicin via 14-3-3γ/Bcl-2

Figure 3

TMP-treated protects VECs’ function against the endotheliotoxicity by Dox in mice. (a) Endothelium-dependent dilation (EDD) of the thoracic aortic strips. (b) Area under of the curve for EDD of the thoracic aortic strips. (c) Western blot’s banding of related proteins in the aortic tissue. (d) Histogram of 14-3-3γ expression in the cytoplasm. (e) Histogram of p-Bad expression in the cytoplasm. (f) Histogram of Bcl-2 expression in the mitochondria. (g) Histogram of p-eNOS/eNOS expression in the cytoplasm. On (c), from left to right, lane 1: control; lane 2: Dox; lane 3: TMP + Dox; lane 4: TMP + Dox + pAD/14-3-3γ-shRNA; lane 5: TMP + Dox + ABT-737. Data are presented as the mean ± SEM. for fifteen individual experiments. a: P < 0.01, versus control group; b: P < 0.01, versus Dox group; c: P < 0.01, versus TMP + Dox group.

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