Tetramethylpyrazine Attenuates the Endotheliotoxicity and the Mitochondrial Dysfunction by Doxorubicin via 14-3-3γ/Bcl-2

Figure 6

TMP-treated preserves the intracellular/mitochondrial balance between ROS generation and their neutralisation in HUVECs. (a) Intracellular/mitochondrial ROS generation of HUVECs after different treatments. Left: Intracellular ROS generation; middle: mitochondrial ROS generation; right: histogram of the intracellular/mitochondrial ROS generation at various treatments. MFI: mean fluorescence intensity. C-ROS: intracellular ROS. M-ROS: mitochondrial ROS. (b) mitochondrial nicotinamide adenine dinucleotide levels of HUVECs after different treatments. Left: histogram of mitochondrial NAD⁺ levels at various treatments; middle: histogram of mitochondrial NADH levels at various treatments; right: histogram of mitochondrial NAD⁺/NADH ratio at various treatments. (c) Intracellular glutathione levels of HUVECs after different treatments. left: histogram of intracellular GSH levels at various treatments; middle: histogram of intracellular GSSG levels at various treatments; right: histogram of intracellular GSH/GSSG ratio at various treatments. Data are presented as the mean ± SEM for eight individual experiments. a: P < 0.01, versus control group; b: P < 0.01, versus Dox group; c: P < 0.01, versus TMP + Dox group.

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