Tetramethylpyrazine Attenuates the Endotheliotoxicity and the Mitochondrial Dysfunction by Doxorubicin via 14-3-3γ/Bcl-2

Figure 8

TMP-treated maintains mitochondrial function in HUVECs. (a) MMP levels were evaluated by JC-1. The results of the fluorescence peak on the x-axis was used to assess the level of MMP. (b) Ca²⁺-induced swelling of mitochondria was used to determine mPTP opening. The changes in absorbance at 520 nm were detected every 2 minute. The data were accessed by the following equation: ΔOD = A520_0min-A520 _20min. (c) Western blot analysis and histogram of cyt C expression in the cytosol. From left to right, lane 1: control; lane 2: Dox; lane 3: TMP + Dox; lane 4: CsA + Dox; lane 5: MitoQ+Dox; lane 6: TMP + Dox + Atr. Data are presented as the mean ± SEM. for eight individual experiments. a: P < 0.01, versus control group; b: P < 0.01, versus Dox group; c: P < 0.01, versus TMP + Dox group.

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