Tetramethylpyrazine Attenuates the Endotheliotoxicity and the Mitochondrial Dysfunction by Doxorubicin via 14-3-3γ/Bcl-2

Table 1

TMP preserved the ferric reducing antioxidant power, the activities of antioxidant enzymes and reduced the levels of lipid peroxidation in the mice’s serum against Dox’s endotheliotoxicity.

Groups	FRAP (mmol Fe^2+/L)	SOD activity (U/g protein)	GSH-Px activity (U/g protein)	CAT activity (U/g protein)	MDA content (nmol/g protein)
Control	4.92 ± 0.21	96.1 ± 5.2	26.3 ± 1.8	17.5 ± 1.1	33.2 ± 2.0
Dox	1.56 ± 0.06 ^a	22.8 ± 1.9 ^a	5.2 ± 0.3 ^a	4.7 ± 0.4 ^a	151.6 ± 14.1 ^a
TMP + dox	3.60 ± 0.23 ^b	66.1 ± 3.9 ^b	17.1 ± 1.5 ^b	13.6 ± 1.2 ^b	55.7 ± 5.1 ^b
TMP + dox + pAD/14-3-3γ-shRNA	1.85 ± 0.07 ^b,c	27.3 ± 2.4 ^b,c	8.2 ± 0.5 ^b,c	7.6 ± 0.6 ^b,c	115.2 ± 12.2 ^b,c
TMP + dox + ABT-737	1.95 ± 0.09 ^b,c	29.2 ± 3.1 ^b,c	9.1 ± 0.8 ^b,c	7.9 ± 0.8 ^b,c	102.5 ± 11.5 ^b,c

Values were presented as mean ± SEM. for fifteen individual experiments. a: P < 0.01, versus control group; b: P < 0.01 versus Dox group; c: P < 0.01 versus TMP + Dox group.