Sedoheptulose-1,7-bisphospate Accumulation and Metabolic Anomalies in Hepatoma Cells Exposed to Oxidative Stress

Figure 1

Early changes in metabolism of H₂O₂-treated hepatoma cells. Cells were treated with 5 mM H₂O₂ for 0, 15, 30, 60, 90, and 120 min and harvested for metabolomic analysis. Molecular features were identified by Progenesis QI, and the data were processed and analyzed using SIMCA-P. (a) Orthogonal partial least squares discriminant analysis (OPLS-DA) score plot for various treatment groups are shown. (b) OPLS-DA score plot of the 0 min- and 15 min-treated groups. The ellipse shown in the model (a, b) represents the Hotelling’s with 95% confidence. (c) Variable importance in projection (VIP) plot of the OPLS-DA model for the 0 min- and 15 min-treated groups. Selected metabolites with a VIP are presented. (d) The data were subjected to metabolite pathway analysis. A summary plot for metabolite set enrichment analysis (MSEA), in which metabolite sets are ranked according to the value, is shown. The bar plot is color coded according to the calculated values.

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