Carnosol Improved Lifespan and Healthspan by Promoting Antioxidant Capacity in Caenorhabditis elegans

Figure 6

Mechanism of the carnosol-induced effects on antioxidant defense and longevity in C. elegans. (a) qRT-PCR was performed to investigate the expression of hsp-16.1, hsp-16.2, sod-3, sod-5, ctl-1, ctl-2, daf-2, daf-16, and hsf-1. (b) SOD-3::GFP expression in carnosol-treated worms was higher than that in control worms. (c) Carnosol treatment caused lifespan extension in the daf-16(mgDf50) mutant. (d) Representative images of the transgenic strain TJ356 with cytosolic, intermediate, and nuclear DAF-16::GFP localization. (e) Effect of carnosol on subcellular DAF-16 localization. (f) Carnosol did not extend lifespan in the hsf-1(sy441) I mutant. (g) Model of action of the carnosol-mediated lifespan extension in C. elegans.

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