HIV-1 Reverse Transcriptase Promotes Tumor Growth and Metastasis Formation via ROS-Dependent Upregulation of Twist

Figure 4

Performance of the derivatives of 4T1luc2 cells expressing variants of consensus HIV-1 FSU_A reverse transcriptase (Table 2) in the wound healing assay. (a) The length of the gap (wound) in μm, at the time of the scratch and 14 and 18 hours later. (b) Distance covered by the cells during the first 14 hours after the scratch, calculated as a difference in the length of the gap at the time of the scratch and after 14 hours. (c) Distance covered by the cells in the interval between 14 and 18 hours after the scratch. (d) Speed of cell migration during the first 14 hours after the scratch, calculated as the covered distance divided by 14 h. (e) Speed of cell migration in the interval between 14 and 18 hours after the scratch. The length of the gap (distance covered by the cells) was calculated based on the light microscopy images using ImageJ software. All values are expressed as from five independent experiment runs. ns: not significant; ; ; ; .

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