Higd1a Protects Cells from Lipotoxicity under High-Fat Exposure

Figure 1

Higd1a expression is elevated under high-fat exposure. (a) LO2 cells were treated with various concentrations of oleic acid and palmitate (2 : 1) for 72 hours, and qRT-PCR and western blot (b) revealed that Higd1a expression was increased significantly upon exposure to 0.4 mM OA and 0.2 mM palmitate. (c) Cells were treated with 0.4 mM palmitate or untreated, and CCK8 assay revealed inhibition of cell proliferation in the FFA group. (d) Cell apoptosis was measured by flow cytometry using Annexin V/PI staining in the abovementioned groups, and more apoptosis was induced in the FFA group; LO2 cells were treated with 0.4 mM OA (e, f) or 0.4 mM palmitate (g, h) alone, and qRT-PCR and western blot were used to detect Higd1a expression in each group; Higd1a mRNA and protein expression in the livers of NAFLD patients was measured by qRT-PCR (i) and western blot (j) ( compared with the control group).

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