Higd1a Protects Cells from Lipotoxicity under High-Fat Exposure

Figure 3

Overexpression of Higd1a protects mitochondrial transmembrane potential under high-fat exposure and vice versa. (a) Mitochondrial transmembrane potential (MMP) in the control group, FFA group, FFA+pcDNA NC group, and FFA+pcDNA Higd1a group was measured by fluorescence microscopy (Olympus, Japan) (JC-1 dye was used to evaluate MMP; red fluorescence indicated cells with normal MMP, and green fluorescence indicated cells with impaired MMP), and the impairment of MMP was less severe in the FFA+pcDNA Higd1a group compared with the FFA+pcDNA NC group. (b) MMP in the control group, FFA group, FFA+siRNA NC group, and FFA+siRNA Higd1a group was measured by fluorescence microscopy, and the impairment of MMP was more severe in the FFA+siRNA Higd1a group compared with the FFA+siRNA NC group ( compared with the control group; compared with the FFA+pcDNA NC group; compared with the FFA+siRNA NC group).

(a)

(b)