Research Article
Higd1a Protects Cells from Lipotoxicity under High-Fat Exposure
Figure 4
ROS promote Higd1a expression under high-fat exposure. (a) Flow cytometry using DCFH-DA identified intracellular ROS production in the control group, FFA group, and FFA+NAC group, and intracellular ROS was significantly elevated in the FFA group. (b) qRT-PCR and western blot (c) detected Higd1a expression in the abovementioned groups, and Higd1a expression was elevated in the FFA group but decreased in the FFA+NAC group. (d) qRT-PCR and western blot (e) detected the expression of Higd1a in the control group, H2O2 group, and the H2O2+NAC group, and Higd1a expression was increased in the H2O2 group but decreased in the H2O2+NAC group ( compared with the control group; compared with the FFA group; compared with the H2O2 group).
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