Higd1a Protects Cells from Lipotoxicity under High-Fat Exposure

Figure 5

ROS promote Higd1a expression by upregulating HIF-1a. (a) HIF-1a expression in the control group, FFA group, and FFA+NAC group was measured by qRT-PCR and western blot (b), and HIF-1a expression was elevated in the FFA group but decreased in the FFA+NAC group. (c) Cells were transfected with siRNA NC or siRNA HIF-1a and then treated with FFAs; qRT-PCR and western blot (d) were used to detect Higd1a expression in each group, and Higd1a expression was decreased in the FFA+siRNA HIF-1a group compared with the FFA+siRNA NC group. (e) Cells were transfected with pcDNA NC or pcDNA HIF-1a and then treated with FFAs, and qRT-PCR and western blot (f) demonstrated that Higd1a expression was increased in the FFA+pcDNA HIF-1a group compared with the FFA+pcDNA NC group. (g) Cells were transfected with siRNA NC or siRNA HIF-1a and then treated with H₂O₂, and qRT-PCR and western blot (h) revealed that HIF-1a and Higd1a expressions were increased in the H₂O₂+siRNA NC group but decreased in the H₂O₂+siRNA HIF-1a group ( compared with the control group; compared with the FFA group; compared with the FFA+siRNA NC (pcDNA NC) group; compared with the H₂O₂+siRNA HIF-1a group).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)