Mechanism of KLF4 Protection against Acute Liver Injury via Inhibition of Apelin Signaling

Figure 1

Differential expression of apelin and KLF4 in CCl₄-induced acute liver injury. Mice received an intraperitoneal injection of carbon tetrachloride (0.2 mL CCl₄ dissolved in 100 mL olive oil) at a dose of 10 mL/kg body weight. The mice in the control group were injected with oil only. (a) Representative photomicrographs of liver histology (HE staining; magnification: upper ×200; lower ×400). (b, c) Serum ALT and AST levels. compared with the vehicle (Veh) group. (d) Immunohistochemical staining of apelin and KLF4 protein following to olive oil and CCl₄ treatment (magnification: upper ×200; lower ×400); (e–h) mRNA expression of apelin, KLF4, TNF-α, and cyclinD1 in CCl₄-induced mice. ^# or compared with the Veh group. (i) Protein expression of apelin, KLF4, and cyclin D1 in CCl₄-induced mice. β-Actin was used as a loading control.

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