Protective Effect of Fat Extract on UVB-Induced Photoaging In Vitro and In Vivo

Figure 2

Effects of FE on SA-β-gal staining and morphology of human skin fibroblasts. Human skin fibroblasts were pretreated with or without FE (0%, 1%, 5%, and 10%) for 24 h, and the cells were then exposed to UVB light at a total dose of 100 mJ/cm². (a) SA-β-gal-positive cells were present 72 h after UVB irradiation. Scale bar: 150 μm. (b) The percentage of SA-β-gal-positive aged cells was determined by counting 200 cells per field. (c) Fluorescent labeling of cytoskeletal proteins. Scale bar: 100 μm. (d) The length/width ratio of cells was determined after staining for cytoskeletal proteins. FE treatment significantly reduced the number of SA-β-gal-positive cells induced by UVB irradiation and preserved normal cell morphology ().

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