Protective Effect of Fat Extract on UVB-Induced Photoaging In Vitro and In Vivo

Figure 3

FE reduced UVB-induced intracellular ROS and promoted the expression of GPX-1 and COL-1 in human skin fibroblasts. Human skin fibroblasts were pretreated with or without FE (0%, 1%, 5%, and 10%) for 24 h, and the cells were then exposed to UVB light at a total dose of 100 mJ/cm². (a) Intracellular ROS measured by DCFH₂-DA staining 20 min after UVB irradiation. Scale bar: 500 μm. (b) Intracellular ROS detected using DCFH₂-DA staining were quantified with flow cytometry. (c, d) Western blotting analysis of GPX-1, catalase, SOD-2, and SOD-1 72 h after UVB irradiation. Representative results from three independent experiments are presented. The density values are expressed in arbitrary units. (e, f) Western blot analysis of COL-1 levels 72 h after UVB irradiation. Representative results from three independent experiments are presented. The density values are expressed in arbitrary units ().

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