Research Article
HB-EGF Ameliorates Oxidative Stress-Mediated Uterine Decidualization Damage
Figure 4
Effects of HB-EGF on intracellular O2− level in H2O2-treated stromal cells. (a) Real-time PCR analysis of HB-EGF mRNA expression after treatment with H2O2 or both H2O2 and NAC. (b) ELISA analysis of HB-EGF protein level after treatment with H2O2 or both H2O2 and NAC. (c and d) HB-EGF hindered the induction of H2O2 on intracellular O2− level. After treatment with rHB-EGF and then exposure to H2O2, the intracellular O2− level was analyzed by flow cytometry during in vitro decidualization. NS: not significant.
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