HB-EGF Ameliorates Oxidative Stress-Mediated Uterine Decidualization Damage

Figure 6

HB-EGF prevents mitochondrial dysfunction in H₂O₂-treated stromal cells. (a) NAC and HB-EGF impeded the impairment of H₂O₂ on mtDNA copy number in stromal cells during in vitro decidualization. After treatment with NAC or rHB-EGF and then addition of H₂O₂, mtDNA copy number was determined by real-time PCR. (b) NAC and HB-EGF blocked the impairment of H₂O₂ on ATP level in stromal cells during in vitro decidualization. (c and d) Flow cytometry analysis of mitochondrial membrane potential after treatment with NAC or rHB-EGF and then addition of H₂O₂ during in vitro decidualization.

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