DPP4 Inhibitor Attenuates Severe Acute Pancreatitis-Associated Intestinal Inflammation via Nrf2 Signaling

Figure 1

Sitagliptin protects IEC6 cells stimulated by LPS. (a) Cell viability was measured by RTCA cell proliferation detection exposure to various concentrations of LPS that range from 1 μg/ml to 100 μg/ml. (b) Cell proliferation detection by RTCA exposure to LPS 10 μg/ml, plus sitagliptin (100 μM) or none. (c) Real-time PCR results of IL-1β, IL-6, and TNF-α in IEC6 cells after incubating in LPS (10 μg/ml) plus sitagliptin (100 μM) or none for 24 h. (d) Western blotting results of IL-1β, IL-6, and TNF-α in IEC-6 cells after incubating in LPS (10 μg/ml) plus sitagliptin (100 μM) or none for 24 h. (e) The fluorescence intensity of ROS (red) after culturing with LPS (10 μg/ml) media plus sitagliptin (100 μM) or none for 30 min was detected using Leica TCS SP8 (×400). Cell nuclei were counterstained with DAPI (blue). Data are presented as error of the mean (SEM) (). , compared with the N group. , compared with the LPS group. N, the control group; SIT, the 100 μM sitagliptin group; LPS, the 10 μg/ml lipopolysaccharide group; LPS+SIT, the 10 μg/ml lipopolysaccharide+100 μM sitagliptin group.

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