DPP4 Inhibitor Attenuates Severe Acute Pancreatitis-Associated Intestinal Inflammation via Nrf2 Signaling

Figure 4

The anti-inflammatory effect of sitagliptin was significantly reduced in Nrf2^-/- mice. Nrf 2^-/- mice received sitagliptin (100 or 200 mg/kg, IP) or vehicle 1 h prior to cerulein+LPS (SAP model). The serum, pancreas, and intestine tissues were then harvested 24 h after the last intraperitoneal injection. (a) DPP4 activity in serum. (b) The cytokines’ IL-6 in serum. (c) The cytokines’ IL-1β in serum. (d) Representative hematoxylin and eosin- (H&E-) stained pancreas and intestine sections (×200). (e) The pancreatitis score. (f) Histological scores to evaluate the degree of injury. Slides were evaluated by two independent investigators in a blinded manner. (g) Concentration of MDA in intestinal tissues from the different groups. (h) Activity of SOD in intestinal tissues from the different groups. Data are presented as error of the mean (SEM) (). , compared with the N group. , compared with the SAP group. N, the control group; SAP, the severe acute pancreatitis group; SAP+SIT 100, the severe acute pancreatitis+100 μM sitagliptin group; SAP+SIT 200, the severe acute pancreatitis+200 μM sitagliptin group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)