Bucindolol Modulates Cardiac Remodeling by Attenuating Oxidative Stress in H9c2 Cardiac Cells Exposed to Norepinephrine

Figure 1

Western blot of proteins involved in cell survival and cell death signaling pathway. H9c2 cardiac cells were incubated with norepinephrine (10 μM) during a period of 24 h, followed by 8 h of bucindolol (10 μM) treatment. β-Actin was used for normalization. Immunoblot of p-STAT3/total-STAT3 (a), PI3K (b), p-Akt/total Akt (c), p-mTOR/mTOR (d), p70 S6K (e), p-AMPK/total AMPK (f), and LC3-II/LC3-I (g) was determined. Values were expressed as from 4 independent experiments. Two-way ANOVA followed by Tukey test: ^A vs. Control Untreated (CTL), ^B vs. Norepinephrine Untreated (NE), ^C vs. Control+Bucindolol (CTL+BCD), ⁺ CTL and CTL+BCD vs. NE and Norepinephrine+Bucindolol (NE+BCD), and CTL+BCD and NE+BCD vs. CTL and NE.

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