Bucindolol Modulates Cardiac Remodeling by Attenuating Oxidative Stress in H9c2 Cardiac Cells Exposed to Norepinephrine

Figure 4

Oxidative stress analysis in H9c2 cardiac cells incubated with norepinephrine (10 μM) for 24 hours, followed by 8 hours of bucindolol (10 μM) treatment. ROS was determined by the CM-H₂DCFDA assay and measured by flow cytometry (c). Representative dot plots indicating the cell populations of all groups (a). Representative histogram indicating cell counts of ROS levels versus FL1-H fluorescence (b). Values expressed as from 3 independent experiments. Two-way ANOVA followed by Tukey: ^B vs. Norepinephrine Untreated (NE), Control+Bucindolol (CTL+BCD) and Norepineprine+Bucindolol (NE+BCD) vs. Control Untreated (CTL) and NE.

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