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Figure 1: Effect of various concentration BA6 on mitochondrial function in A549 cells. (a) The mitochondrial respiration assay started with four basal OCR measurements, followed by continuous injections of oligomycin, FCCP, and rotenone. The basic parameters measured include basal respiration, ATP production, proton leak, maximum respiration, and spare respiratory capacity. (b) Time course and OCR curve plot. OCR in basal and BA6 treatment conditions (0.1, 1, and 10 μM for 24 h) were examined by the Seahorse XF24 analyzer in the absence or presence of oligomycin (1 μM), FCCP (0.25 μM), and rotenone (1 μM). Quantification of changes in parameters induced by BA6 treatment includes the basal respiration (c), ATP production (d), proton leak (e), maximum respiration (f), spare respiratory capacity (g), and ECAR (i). OCR and ECAR levels are quantified by normalization of cell numbers. Data were obtained from three independent experiments and expressed as the . and , as compared with untreated cells.