TRPA1 Promotes Cardiac Myofibroblast Transdifferentiation after Myocardial Infarction Injury via the Calcineurin-NFAT-DYRK1A Signaling Pathway

Figure 3

Deletion of TRPA1 in resident CFs abrogates TGF-β-mediated CMF transdifferentiation. (a and b) Immunofluorescence staining (a) and quantification (b) of α-SMA-positive (red) stress fibers and DAPI (blue) in neonatal Trpa1^+/+ and Trpa1^-/- mouse primary CFs with and without TGF-β stimulation (scale  μm). (c and d) Images (c) and quantification (d) of the contraction of floating collagen gel matrices seeded with Trpa1^+/+ or Trpa1^-/- CFs at 24 hr after TGF-β stimulation. The white arrows show the direction of contraction. per group. (e and f) Western blot analysis and quantification of Postn protein expression in primary Trpa1^+/+ and Trpa1^-/- CFs, with and without TGF-β stimulation. Molecular weights in kDa are shown to the right of the blots. per group. The error bars represent the and . The data are representative of the results of three or more independent experiments.

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