TRPA1 Promotes Cardiac Myofibroblast Transdifferentiation after Myocardial Infarction Injury via the Calcineurin-NFAT-DYRK1A Signaling Pathway

Figure 4

Overexpression of TRPA1 in primary CFs enhances CMF transdifferentiation. (a) Western blot analysis and quantification of TRPA1 protein expression in neonatal WT mouse primary CFs with and without TGF-β stimulation. Molecular weights in kDa are shown to the right of the blots. per group. (b and c) Immunofluorescence staining (b) and quantification (c) of α-SMA-positive (red) stress fibers and DAPI (blue) in CFs treated with TGF-β transfected with negative control (NC) adenoviral vector (not shown), Ad-TRPA1 (Ad), negative control (NC) siRNA (not shown), or si-TRPA1 (si) and treated 48 hr later with TGF-β. (scale  μm). per group. (d) α-SMA-luciferase promoter activity in CFs treated as described above. per group. (e) Western blot analysis and quantification of Postn protein expression in CFs treated as described above. Molecular weights in kDa are shown to the right of the blots. per group. (f and g) Images (f) and quantification (g) of the contraction of floating collagen gel matrices seeded with CFs treated as described above. per group. The error bars represent the and . The data are representative of the results of three or more independent experiments.

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